Journal Article

Transcriptional and metabolic profiling of grape (<i>Vitis vinifera</i> L.) leaves unravel possible innate resistance against pathogenic fungi

Andreia Figueiredo, Ana Margarida Fortes, Silvia Ferreira, Mónica Sebastiana, Young Hae Choi, Lisete Sousa, Bartolomeu Acioli-Santos, Fernando Pessoa, Robert Verpoorte and Maria Salomé Pais

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 59, issue 12, pages 3371-3381
Published in print September 2008 | ISSN: 0022-0957
Published online July 2008 | e-ISSN: 1460-2431 | DOI:
Transcriptional and metabolic profiling of grape (Vitis vinifera L.) leaves unravel possible innate resistance against pathogenic fungi

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Grapevine species (Vitis sp.) are prone to several diseases, fungi being the major pathogens compromising its cultivation and economic profit around the world. Knowledge of the complexity of mechanisms responsible for resistance to fungus infection of cultivars, such as Regent, is necessary for strategies to be defined which will improve resistance in highly susceptible crop species. Transcript and metabolic profiles of the Vitis vinifera cultivars Regent and Trincadeira (resistant and susceptible to fungi, respectively) were analysed by cDNA microarray, quantitative real-time PCR, and nuclear magnetic resonance spectroscopy. The integration of datasets obtained through transcriptome and metabolome analysis revealed differences in transcripts and metabolites between both cultivars. These differences are probably associated with the innate resistance of Regent towards the mildews. Several transcripts related to stress and defence, namely a subtilisin-like protease, phenylalanine ammonia lyase, S-adenosylmethionine synthase, WD-repeat protein like, and J2P, were up-regulated in Regent suggesting an intrinsic resistance capability of this cultivar. A metabolic profile revealed an accumulation of compounds such as inositol and caffeic acid, which are known to confer resistance to fungi. The differences in transcripts and metabolites detected are discussed in terms of the metabolic pathways and their possible role in plant defence against pathogen attack, as well as their potential interest to discriminate among resistant and susceptible grapevine cultivars.

Keywords: cDNA microarrays; NMR-based metabolomics; Regent; Vitis vinifera

Journal Article.  6146 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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