Journal Article

Real-time electrochemical detection of extracellular nitric oxide in tobacco cells exposed to cryptogein, an elicitor of defence responses

Angélique Besson-Bard, Sophie Griveau, Fethi Bedioui and David Wendehenne

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 59, issue 12, pages 3407-3414
Published in print September 2008 | ISSN: 0022-0957
Published online July 2008 | e-ISSN: 1460-2431 | DOI: https://dx.doi.org/10.1093/jxb/ern189

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It was previously reported that cryptogein, an elicitor of defence responses, induces an intracellular production of nitric oxide (NO) in tobacco. Here, the possibility was explored that cryptogein might also trigger an increase of NO extracellular content through two distinct approaches, an indirect method using the NO probe 4,5-diaminofluorescein (DAF-2) and an electrochemical method involving a chemically modified microelectrode probing free NO in biological media. While the chemical nature of DAF-2-reactive compound(s) is still uncertain, the electrochemical modified microelectrodes provide real-time evidence that cryptogein induces an increase of extracellular NO. Direct measurement of free extracellular NO might offer important new insights into its role in plants challenged by biotic stresses.

Keywords: Diaminofluorescein; diethylamine NONOate; electrochemical sensor; nitric oxide; plant defence responses

Journal Article.  5232 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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