Journal Article

Gene expression and sensitivity in response to copper stress in rice leaves

Emi Sudo, Misao Itouga, Kayo Yoshida-Hatanaka, Yoshiro Ono and Hitoshi Sakakibara

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 59, issue 12, pages 3465-3474
Published in print September 2008 | ISSN: 0022-0957
Published online August 2008 | e-ISSN: 1460-2431 | DOI: http://dx.doi.org/10.1093/jxb/ern196

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Gene expression in response to Cu stress in rice leaves was quantified using DNA microarray (Agilent 22K Rice Oligo Microarray) and real-time PCR technology. Rice plants were grown in hydroponic solutions containing 0.3 (control), 10, 45, or 130 μM of CuCl2, and Cu accumulation and photosynthesis inhibition were observed in leaves within 1 d of the start of treatment. Microarray analysis flagged 305 Cu-responsive genes, and their expression profile showed that a large proportion of general and defence stress response genes are up-regulated under excess Cu conditions, whereas photosynthesis and transport-related genes are down-regulated. The Cu sensitivity of each Cu-responsive gene was estimated by the median effective concentration value (EC50) and the range of fold-changes (F) under the highest (130 μM) Cu conditions (|log2F|130). Our results indicate that defence-related genes involved in phytoalexin and lignin biosynthesis were the most sensitive to Cu, and that plant management of abiotic and pathogen stresses has overlapping components, possibly including signal transduction.

Keywords: Copper-sensitivity; DNA microarray; excess copper stress; gene expression; Oryza sativa L

Journal Article.  4689 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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