Journal Article

Expression and regulation of prostaglandin E synthase isoforms in human myometrium with labour

S. Astle, R. Newton, S. Thornton, M. Vatish and D.M. Slater

in MHR: Basic science of reproductive medicine

Published on behalf of European Society of Human Reproduction and Embryology

Volume 13, issue 1, pages 69-75
Published in print January 2007 | ISSN: 1360-9947
Published online November 2006 | e-ISSN: 1460-2407 | DOI: http://dx.doi.org/10.1093/molehr/gal093
Expression and regulation of prostaglandin E synthase isoforms in human myometrium with labour

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Since the controversies regarding the use of non-steroidal anti-inflammatory drugs (NSAIDs) and selective cyclo-oxygenase (COX)-2 antagonists for the treatment of preterm labour (PTL), more emphasis has been placed on investigating the terminal synthases involved in the production of prostaglandins (PGs) to allow more targeted therapy in PTL. Prostaglandin E2 (PGE2) is synthesized by one of three enzymes, cytosolic prostaglandin E synthase (cPGES), microsomal PGES-1 (mPGES-1) and microsomal PGES-2 (mPGES-2). We have determined (i) the immuno-localization of all three PGES enzymes in lower segment pregnant human myometrium, (ii) the expression of PGES and COX-2 mRNA expression at term and preterm gestation with and without labour and (iii) the effect of interleukin (IL)-1β on COX-2 and PGES mRNA and protein expression in human myometrial smooth muscle (HMSM) cell cultures. We show mPGES-1 protein located predominantly in myometrial and vascular smooth muscle cells (SMCs), whilst mPGES-2 protein is largely in stromal cells surrounding the SMC and cPGES is diffusely located throughout the myometrium. Expression of mPGES-2 mRNA increased with term labour and PTL and expression of COX-2 and mPGES-1 mRNA with term labour, whereas cPGES expression did not change. IL-1β stimulated release of PGE2 by HMSM cells and increased COX-2 and mPGES-1 mRNA and protein expression. Thus, COX-2 expression and mPGES-1 expression are co-ordinately up-regulated in lower segment myometrium with term labour and with IL-1β treatment in HMSM cells.

Keywords: prostaglandin E2 synthase; myometrium; labour; myometrial smooth muscle

Journal Article.  6204 words.  Illustrated.

Subjects: Reproductive Medicine

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