Journal Article

Ethylene Controls Autophosphorylation of the Histidine Kinase Domain in Ethylene Receptor ETR1

Jan Voet-van-Vormizeele and Georg Groth

in Molecular Plant

Published on behalf of IPPE, SIBS, CAS and Chinese Society for Plant Biology (CSPB)

Volume 1, issue 2, pages 380-387
Published in print March 2008 | ISSN: 1674-2052
Published online February 2008 | e-ISSN: 1752-9867 | DOI: http://dx.doi.org/10.1093/mp/ssn004
Ethylene Controls Autophosphorylation of the Histidine Kinase Domain in Ethylene Receptor ETR1

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  • Molecular and Cell Biology
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Perception of the phytohormone ethylene is accomplished by a small family of integral membrane receptors. In Arabidopsis, five ethylene receptor proteins are known, including ethylene resistant 1 (ETR1). The hydrophobic amino-terminal domain of these receptors contains the ethylene-binding site while the carboxyl-terminal part consists of a histidine kinase domain and a response regulator domain, which are well known elements found in bacterial two-component signaling. The soluble membrane-extrinsic carboxyl-terminal part of the receptor, which is likely to play an important role in signal transduction, showed intrinsic kinase activity when expressed and purified on its own. However, a correlation between signal input and autokinase activity was not established in these studies, as receptors were missing the transmembrane amino-terminal sensor domain. Thus, it is still unclear whether autophosphorylation occurs in response to perception of the ethylene signal. Here, we report on autophosphorylation studies of purified full-length ETR1. Autokinase activity of the purified receptor is controlled by ethylene or by ethylene agonists like the π-acceptor compound cyanide. In fact, both signal molecules were able to completely turn off the intrinsic kinase activity. Furthermore, the observed inhibition of autophosphorylation in ETR1 by both molecules could be prevented when the ethylene antagonist 1-methyl-cyclopropene (MCP) was applied.

Keywords: ethylene pathway; phytohormone receptor; membrane protein; two-component system; signal transduction; Arabidopsis thaliana

Journal Article.  5040 words.  Illustrated.

Subjects: Molecular and Cell Biology ; Plant Sciences and Forestry

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