Journal Article

Identification of Quantitative Trait Loci Affecting Hemicellulose Characteristics Based on Cell Wall Composition in a Wild and Cultivated Rice Species

Si-Ju Zhang, Xue-Qin Song, Bai-Sheng Yu, Bao-Cai Zhang, Chuan-Qing Sun, J. Paul Knox and Yi-Hua Zhou

in Molecular Plant

Published on behalf of IPPE, SIBS, CAS and Chinese Society for Plant Biology (CSPB)

Volume 5, issue 1, pages 162-175
Published in print January 2012 | ISSN: 1674-2052
Published online September 2011 | e-ISSN: 1752-9867 | DOI: https://dx.doi.org/10.1093/mp/ssr076
Identification of Quantitative Trait Loci Affecting Hemicellulose Characteristics Based on Cell Wall Composition in a Wild and Cultivated Rice Species

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  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Cell wall hemicellulosic polysaccharides are structurally complex and diverse. Knowledge about the synthesis of cell wall hemicelluloses and their biological roles is limited. Quantitative trait loci (QTL) mapping is a helpful tool for the dissection of complex phenotypes for gene identification. In this study, we exploited the natural variation in cell wall monosaccharide levels between a common wild rice, Yuanj, and an elite indica cultivar, Teqing, and performed QTL mapping with their introgression lines (ILs). Chemical analyses conducted on the culms of Yuanj and Teqing showed that the major alterations are found in glucose and xylose levels, which are correlated with specific hemicellulosic polymers. Glycosidic linkage examination revealed that, in Yuanj, an increase in glucose content results from a higher level of mixed linkage β-glucan (MLG), whereas a reduction in xylose content reflects a low level of xylan backbone and a varied arabinoxylan (AX) structure. Seventeen QTLs for monosaccharides have been identified through composition analysis of the culm residues of 95 core ILs. Four major QTLs affecting xylose and glucose levels are responsible for 19 and 21% of the phenotypic variance, respectively. This study provides a unique resource for the genetic dissection of rice cell wall formation and remodeling in the vegetative organs.

Keywords: Quantitative trait loci; cell wall composition; hemicellulose; rice introgression lines; wild rice species

Journal Article.  7095 words.  Illustrated.

Subjects: Molecular and Cell Biology ; Plant Sciences and Forestry

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