Journal Article

Synthesis and Properties of DNA-PNA Chimeric Oligomers

Patrick J. Finn, Neil J. Gibson, Rachel Fallon, Alan Hamilton and Tom Brown

in Nucleic Acids Research

Volume 24, issue 17, pages 3357-3363
Published in print September 1996 | ISSN: 0305-1048
Published online September 1996 | e-ISSN: 1362-4962 | DOI: http://dx.doi.org/10.1093/nar/24.17.3357
Synthesis and Properties of DNA-PNA Chimeric Oligomers

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Adenine, thymine and cytosine PNA monomers have been prepared using 3-amino-1,2-propanediol as a starting material. The benzoyl group was used to protect the exocyclic amines of the heterocyclic bases of A and C PNA monomers and the backbone primary amine was protected with the monomethoxytrityl group. The thymine and cytosine PNA monomers were used in conjunction with standard DNA synthesis monomers to produce chimeric PNA DNA (PDC) oligomers. Ultraviolet melting studies confirmed that these oligomers form stable hybrids with complementary DNA strands and that mismatches in the DNA but more so in the PNA sections lead to duplex destabilisation.

Journal Article.  4905 words.  Illustrated.

Subjects: Chemistry ; Biochemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology

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