Journal Article

A 43 kDa DNA Binding Protein from the Pea Chloroplast Interacts with and Stimulates the Cognate DNA Polymerase

Weiliang Chen, Amos Gaikwad, Sunil Kumar Mukherjee, Nirupam Roy Choudhary, Dhirendra Kumar and Krishna K. Tewari

in Nucleic Acids Research

Volume 24, issue 20, pages 3953-3961
Published in print October 1996 | ISSN: 0305-1048
Published online October 1996 | e-ISSN: 1362-4962 | DOI: http://dx.doi.org/10.1093/nar/24.20.3953
A 43 kDa DNA Binding Protein from the Pea Chloroplast Interacts with and Stimulates the Cognate DNA Polymerase

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A DNA binding protein with DNA polymerase ‘accessory activity’ has been identified and purified to apparent homogeneity from pea chloroplasts. This protein consists of a single subunit of 43 kDa and binds to DNA regardless of its base sequence and topology. It increases cognate DNA polymerase-primase activity in a dose dependent manner. Using solid phase protein-protein interaction trapping and co-immuno-precipitation techniques, the purified protein was found to associate with the chloroplast DNA polymerase. The chloroplast DNA polymerase also binds directly to the radioiodinated 43 kDa protein. The specific interaction between 43 kDa protein and chloroplast DNA polymerase results in the synthesis of longer DNA chains. The 43 kDa protein, present abundantly in the pea chloroplast, appears to increase processivity of the chloroplast DNA polymerase and may play an important role in the replication of pea chloroplast DNA.

Journal Article.  6663 words.  Illustrated.

Subjects: Chemistry ; Biochemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology

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