Journal Article

Placement of the α-sarcin loop within the 50S subunit: Evidence derived using a photolabile oligodeoxynucleotide probe

Parimi Muralikrishna, Rebecca W. Alexander and Barry S. Cooperman

in Nucleic Acids Research

Volume 25, issue 22, pages 4562-4569
Published in print November 1997 | ISSN: 0305-1048
Published online November 1997 | e-ISSN: 1362-4962 | DOI: http://dx.doi.org/10.1093/nar/25.22.4562
Placement of the α-sarcin loop within the 50S subunit: Evidence derived using a photolabile oligodeoxynucleotide probe

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We report the synthesis of a radioactive, photolabile oligodeoxyribonucleotide probe and its exploitation in identifying 50S ribosomal subunit components neighboring the α-sarcin loop. The probe is complementary to 23S rRNA nt 2653–2674. Photolysis of the complex formed between the probe and 50S subunits leads to site-specific probe photoincorporation into proteins L2, the most highly labeled protein, L1, L15, L16 and L27, labeled to intermediate extents, and L5, L9, L17 and L24, each labeled to a minor extent. Portions of each of these proteins thus lie within 23 Å of nt U2653. These results lead us to conclude that the α-sarcin loop is located at the base of the L1 projection within the 50S subunit. Such placement, near the peptidyl transferase center, provides a rationale for the extreme sensitivity of ribosomal function to cleavage of the α-sarcin loop.

Journal Article.  4915 words.  Illustrated.

Subjects: Chemistry ; Biochemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology

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