Journal Article

Cloning, chromosomal localization and promoter analysis of the human transcription factor YY1

Ya-Li Yao, Subir Ghosh, Yong Fang, Edward Seto, Barbara R. Dupont and Robin J. Leach

in Nucleic Acids Research

Volume 26, issue 16, pages 3776-3783
Published in print August 1998 | ISSN: 0305-1048
Published online August 1998 | e-ISSN: 1362-4962 | DOI: http://dx.doi.org/10.1093/nar/26.16.3776
Cloning, chromosomal localization and promoter analysis of the human transcription factor YY1

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Yin Yang 1 (YY1) is a protein that activates and represses transcription of a large number of cellular and viral genes. In addition, studies suggest that YY1 may play an important role in development and differentiation. Here, we report the isolation and analysis of a YY1 genomic clone from a lambda human liver library. Fluorescence in situ hybridization with the YY1 clone has localized the YY1 gene to chromosome 14 band q32. A major YY1 gene transcription initiation site has been mapped to 478 bp upstream of the ATG translation start site. The proximal promoter contains multiple Sp1 transcription factor binding sites but lacks a consensus TATA or CCAAT box. Transient transfections and detailed deletion analyses localized the promoter to no more than 277 bp upstream from the major transcription start site. Finally, we have found that overexpression of the adenovirus E1A protein represses expression of a reporter gene directed by the YY1 promoter.

Journal Article.  3517 words.  Illustrated.

Subjects: Chemistry ; Biochemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology

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