Journal Article

Manganese citrate improves base-calling accuracy in DNA sequencing reactions using rhodamine-based fluorescent dye-terminators

Christopher Korch and Harry Drabkin

in Nucleic Acids Research

Volume 27, issue 5, pages 1405-1407
Published in print March 1999 | ISSN: 0305-1048
Published online March 1999 | e-ISSN: 1362-4962 | DOI: http://dx.doi.org/10.1093/nar/27.5.1405
Manganese citrate improves base-calling accuracy in DNA sequencing reactions using rhodamine-based fluorescent dye-terminators

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While dideoxy-terminators labeled with rhodamine-based fluorescent dyes provide the most versatile method of automated DNA sequencing, variation in peak heights reduces base-calling accuracy. We describe a simple approach that uses additions of a manganese salt and the metal buffer sodium citrate (MnCit) to overcome this limitation. This modification reduces peak height variability >2-fold and significantly increases the number of accurately read bases in DNA sequences.

Journal Article.  2041 words.  Illustrated.

Subjects: Chemistry ; Biochemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology

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