Journal Article

Bypass of heterology during strand transfer by <i>Saccharomyces cerevisiae</i> Rad51 protein

Victor F. Holmes, Kirsten R. Benjamin, Nancy J. Crisona and Nicholas R. Cozzarelli

in Nucleic Acids Research

Volume 29, issue 24, pages 5052-5057
Published in print December 2001 | ISSN: 0305-1048
Published online December 2001 | e-ISSN: 1362-4962 | DOI: https://dx.doi.org/10.1093/nar/29.24.5052
Bypass of heterology during strand transfer by Saccharomyces cerevisiae Rad51 protein

More Like This

Show all results sharing these subjects:

  • Chemistry
  • Biochemistry
  • Bioinformatics and Computational Biology
  • Genetics and Genomics
  • Molecular and Cell Biology

GO

Show Summary Details

Preview

During recombination-mediated repair of DNA double-strand breaks, strand transfer proteins must distinguish a homologous repair template from closely related genomic sequences. However, some tolerance by strand transfer proteins for sequence differences is also critical: too much stringency will prevent recombination between different alleles of the same gene, but too much tolerance will lead to illegitimate recombination. We characterized the heterology tolerance of Saccharomyces cerevisiae Rad51 by testing bypass of small heterologous inserts in either the single- or double-stranded substrate of an in vitro strand transfer reaction that models the early steps of homologous recombination. We found that the yeast protein is rather stringent, only tolerating heterologies up to 9 bases long. The efficiency of heterology bypass depends on whether the insert is in the single- or double-stranded substrate, as well as on the location of the insert relative to the end of the double-stranded linear substrate. Rad51 is distinct in that it can catalyze strand transfer in either the 3′→5′ or 5′→3′ direction. We found that bypass of heterology was independent of the polarity of strand transfer, suggesting that the mechanism of 5′→3′ transfer is the same as that of 3′→5′ transfer.

Journal Article.  4161 words.  Illustrated.

Subjects: Chemistry ; Biochemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.