Journal Article

E2F-dependent transcription of the <i>raf</i> proto-oncogene during <i>Drosophila</i> development

Eun-Jeong Kwon, Eun-Jin Oh, Young-Shin Kim, Fumiko Hirose, Katsuhito Ohno, Yasuyoshi Nishida, Akio Matsukage, Masamitsu Yamaguchi and Mi-Ae Yoo

in Nucleic Acids Research

Volume 29, issue 8, pages 1808-1814
Published in print April 2001 | ISSN: 0305-1048
Published online April 2001 | e-ISSN: 1362-4962 | DOI: http://dx.doi.org/10.1093/nar/29.8.1808
E2F-dependent transcription of the raf proto-oncogene during Drosophila development

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D-raf, a Drosophila homolog of the raf proto-oncogene, has diverse functions throughout development and is transcribed in a wide range of tissues, with high levels of expression in the ovary and in association with rapid proliferation. The expression pattern resembles those of S phase genes, which are regulated by E2F transcription factors. In the 5′‐flanking region of D-raf, four sequences (E2F sites 1–4) similar to the E2F recognition sequence were found, one of them (E2F site 3) being recognized efficiently by Drosophila E2F (dE2F) in vitro. Transient luciferase expression assays confirmed activation of the D-raf gene promoter by dE2F/dDP. Expression of DraflacZ was greatly reduced in embryos homozygous for the dE2F mutation. These results suggest that dE2F is likely to be an important regulator of D-raf transcription.

Journal Article.  4965 words.  Illustrated.

Subjects: Chemistry ; Biochemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology

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