Journal Article

A novel mechanism of repression of the vascular endothelial growth factor promoter, by single strand DNA binding cold shock domain (Y‐box) proteins in normoxic fibroblasts

Leeanne S. Coles, Peter Diamond, Lidia Lambrusco, Julie Hunter, Julie Burrows, Mathew A. Vadas and Gregory J. Goodall

in Nucleic Acids Research

Volume 30, issue 22, pages 4845-4854
Published in print November 2002 | ISSN: 0305-1048
Published online November 2002 | e-ISSN: 1362-4962 | DOI: http://dx.doi.org/10.1093/nar/gkf615
A novel mechanism of repression of the vascular endothelial growth factor promoter, by single strand DNA binding cold shock domain (Y‐box) proteins in normoxic fibroblasts

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Overexpression of vascular endothelial growth factor (VEGF) is implicated in a number of diseases. It is therefore critical that mechanisms exist to strictly regulate VEGF expression. A hypoxia‐responsive (HR) region of the VEGF promoter which binds the HIF‐1 transcription factor is a target for many signals that up‐regulate VEGF transcription. Repressors targeting the HIF‐1 transcription factor have been identified but no repressors directly binding the HR promoter region had been reported. We now report a novel mechanism of repression of the VEGF HR region involving DNA binding. We find that single strand DNA‐specific cold shock domain (CSD or Y‐box) proteins repress the HR region via a binding site downstream of the HIF‐1 site. The repressor site is functional in unstimulated, normoxic fibroblasts and represents a novel means to prevent expression of VEGF in the absence of appropriate stimuli. We characterized complexes forming on the VEGF repressor site and identified a previously unreported nuclear CSD protein complex containing dbpA. Nuclear dbpA appears to bind as a dimer and we determined a means by which nuclear CSD proteins may enter double strand DNA to bind to their single strand sites to bring about repression of the VEGF HR region.

Journal Article.  8174 words.  Illustrated.

Subjects: Chemistry ; Biochemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology

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