Journal Article

Quadruplex ligands may act as molecular chaperones for tetramolecular quadruplex formation

Anne De Cian and Jean-Louis Mergny

in Nucleic Acids Research

Volume 35, issue 8, pages 2483-2493
Published in print April 2007 | ISSN: 0305-1048
Published online March 2007 | e-ISSN: 1362-4962 | DOI: http://dx.doi.org/10.1093/nar/gkm098
Quadruplex ligands may act as molecular chaperones for tetramolecular quadruplex formation

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G-quadruplexes are a family of four-stranded DNA structures, stabilized by G-quartets, that form in the presence of monovalent cations. Efforts are currently being made to identify ligands that selectively bind to G-quadruplex motifs as these compounds may interfere with the telomere structure, telomere elongation/replication and proliferation of cancer cells. The kinetics of quadruplex–ligands interactions are poorly understood: it is not clear whether quadruplex ligands lock into the preformed structure (i.e. increase the lifetime of the structure by lowering the dissociation constant, koff) or whether ligands actively promote the formation of the complex and act as quadruplex chaperones by increasing the association constant, kon. We studied the effect of a selective quadruplex ligand, a bisquinolinium pyridine dicarboxamide compound called 360A, to distinguish these two possibilities. We demonstrated that, in addition to binding to and locking into preformed quadruplexes, this molecule acted as a chaperone for tetramolecular complexes by acting on kon. This observation has implications for in vitro and in vivo applications of quadruplexes and should be taken into account when evaluating the cellular responses to these agents.

Journal Article.  6223 words.  Illustrated.

Subjects: Chemistry ; Biochemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology

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