Journal Article

Manipulation of gene expression in zebrafish using caged circular morpholino oligomers

Yuan Wang, Li Wu, Peng Wang, Cong Lv, Zhenjun Yang and Xinjing Tang

in Nucleic Acids Research

Volume 40, issue 21, pages 11155-11162
Published in print November 2012 | ISSN: 0305-1048
Published online September 2012 | e-ISSN: 1362-4962 | DOI: http://dx.doi.org/10.1093/nar/gks840

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Morpholino oligomers (MOs) have been widely used to knock down specific genes in zebrafish, but their constitutive activities limit their experimental applications for studying a gene with multiple functions or within a gene network. We report herein a new design and synthesis of caged circular MOs (caged cMOs) with two ends linked by a photocleavable moiety. These caged cMOs were successfully used to photomodulate β-catenin-2 and no tail expression in zebrafish embryos.

Journal Article.  4734 words.  Illustrated.

Subjects: Chemistry ; Biochemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology

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