Journal Article

Novel cyanine-AMP conjugates for efficient 5′ RNA fluorescent labeling by one-step transcription and replacement of [γ-<sup>32</sup>P]ATP in RNA structural investigation

Na Li, Changjun Yu and Faqing Huang

in Nucleic Acids Research

Volume 33, issue 4, pages e37-e37
Published in print February 2005 | ISSN: 0305-1048
Published online February 2005 | e-ISSN: 1362-4962 | DOI: http://dx.doi.org/10.1093/nar/gni036

Show Summary Details

Preview

Two novel fluorescent cyanine-AMP conjugates, F550/570 and F650/670, have been synthesized to serve as transcription initiators under the T7 φ2.5 promoter. Efficient fluorophore labeling of 5′ RNA is achieved in a single transcription step by including F550/570 and F650/670 in the transcription solution. The current work makes fluorescently labeled RNA readily available for broad applications in biochemistry, molecular biology, structural biology and biomedicine. In particular, site-specifically fluorophore-labeled large RNAs prepared by the current method may be used to investigate RNA structure, folding and mechanism by various fluorescence techniques. In addition, F550/570 and F650/670 may replace [γ-32P]ATP to prepare 5′ labeled RNA for RNA structural and functional investigation, thereby eliminating the need for the unstable and radio-hazardous [γ-32P]ATP.

Journal Article.  5398 words.  Illustrated.

Subjects: Research Methods in Life Sciences

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.