Journal Article

Effect of single base insertion into anticodon loop of frameshift suppressor tRNA

Takahiro Hohsaka, Hikaru Taira, Masaharu Fukushima and Masahiko Sisido

in Nucleic Acids Symposium Series

Volume 1, issue 1, pages 189-190
Published in print November 2001 | ISSN: 0261-3166
Published online November 2001 | e-ISSN: 1746-8272 | DOI: http://dx.doi.org/10.1093/nass/1.1.189
Effect of single base insertion into anticodon loop of frameshift suppressor tRNA

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Frameshift suppressor tRNAs containing an additional nucleotide at anticodon loop were prepared and their translational activity was evaluated in an E. coli in vitro translation system. The mutated tRNACCCG was chemically aminoacylated with nitrophenylalanine, and added to the in vitro translation system together with a streptavidin mRNA containing CGGG codon. Western blot analysis indicates that the mutated tRNAs can decode the four-base codon, except 32.1 C and 33.1 G mutants.

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Subjects: Chemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology

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