Journal Article

A new strategy for the synthesis of oligodeoxynucleotides in the phosphoramidite method without base protection via phosphite intermediates

Akihiro Ohkubo, Yusuke Ezawa, Kohji Seio and Mitsuo Sekine

in Nucleic Acids Symposium Series

Volume 2, issue 1, pages 29-30
Published in print November 2002 | ISSN: 0261-3166
Published online November 2002 | e-ISSN: 1746-8272 | DOI: http://dx.doi.org/10.1093/nass/2.1.29
A new strategy for the synthesis of oligodeoxynucleotides in the phosphoramidite method without base protection via phosphite intermediates

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Deoxyadenosine and deoxycytidine have highly nucleophilic amino groups. Therefore, without base protection, the undesired N-phosphitylations of these amino groups occur in the previous phosphoramidite methods. Now, we report that these N-phosphitylations were considerably suppressed in our new coupling strategy using HOBt as a promoter capable of formation of phosphite-type intermediates.

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Subjects: Chemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology

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