Journal Article

Inhibition of restriction enzyme's DNA sequence recognition by PUVA treatment

Fujinori Hanawa, Mamoru Okamoto and G.H. Neil Towers

in Nucleic Acids Symposium Series

Volume 3, issue 1, pages 297-298
Published in print September 2003 | ISSN: 0261-3166
Published online September 2003 | e-ISSN: 1746-8272 | DOI: http://dx.doi.org/10.1093/nass/3.1.297
Inhibition of restriction enzyme's DNA sequence recognition by PUVA treatment

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Applying various restriction enzymes on a specially designed 1.5 kb DNA fragment revealed that the inhibitory effects of PUVA treatment on restriction endonuclease activities are caused by recognition inhibition. In this study, Restriction enzymes which have a 5′-TpA sequence at the cleaving site (Kpn I, Xba I, Pme I, and Dra I), and non-cleaving site (Pac I) in recognition sites, or have two 5′-TpA sequences at the recognition site and a non-specific sequence between recognition and cleaving site (BciV I) were inhibited by PUVA treatment. Most of the other restriction enzymes used in this study which do not have a 5′-TpA sequence at their restriction site were not inhibited by PUVA treatment, although a 5′-TpA sequence is located adjacent (Sma I) or very close (BamH I, Sac I and Pst I) to the recognition and cleaving site for them.

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Subjects: Chemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology

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