Journal Article

Detection of 5-methylcytosine in DNA by photo-functionalized oligodeoxynucleotides possessing 2-methyl-1,4-naphthoquinone chromophore

Hisatsugu Yamada, Kazuhito Tanabe and Sei-ichi Nishimoto

in Nucleic Acids Symposium Series

Volume 49, issue 1, pages 149-150
Published in print September 2005 | ISSN: 0261-3166
Published online September 2005 | e-ISSN: 1746-8272 | DOI: https://dx.doi.org/10.1093/nass/49.1.149
Detection of 5-methylcytosine in DNA by photo-functionalized oligodeoxynucleotides possessing 2-methyl-1,4-naphthoquinone chromophore

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We developed a method for detection of 5-methylcytosine in DNA by photosensitized oxidation, using photo-functionalized oligodeoxynucleotides possessing 2-methyl-1,4-naphthoquinone chromophores (NQ) at the interior of the strand. The photooxidation and subsequent hot piperidine treatment of duplex consisting of the NQ-tethered oligodeoxynucleotide and its complementary strand bearing 5-methylcytosine residue led to selective DNA strand cleavage at the 5-methylcytosine residue, whereas no strand cleavage was detected for the control duplex with normal cytosine residue but not 5-methylcytosine.

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Subjects: Chemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology

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