Journal Article

Development of an N-unprotected phosphoramidite method for the chemical synthesis of aminoacylated RNAs

Akihiro Ohkubo, Yasuhiro Noma, Haruhiko Taguchi, Kohji Seio and Mitsuo Sekine

in Nucleic Acids Symposium Series

Volume 51, issue 1, pages 1-2
Published in print November 2007 | ISSN: 0261-3166
Published online November 2007 | e-ISSN: 1746-8272 | DOI: http://dx.doi.org/10.1093/nass/nrm001
Development of an N-unprotected phosphoramidite method for the chemical synthesis of aminoacylated RNAs

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In the previous study, we developed a method for O-selective phosphorylation, i.e., "the activated phosphite method" for DNA synthesis without base protection. However, the O-selectivity was low in RNA synthesis when the activated phosphite method was used. In this paper, we developed a new method for the O-selective phosphorylation available for the chemical synthesis of aminoacylated RNAs on polymer supports. As the result, it was found that the selectivity of the phosphorylation in RNA synthesis without base protection increased to more than 99% by treatment of the undesired N-P(III) bonds with 1-hydroxyl-6-nitorobenzotriazole, independent of the kind of protecting groups at the 2′ position. Moreover, we succeeded in synthesizing a 21 mer oligoRNA without base protection.

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Subjects: Chemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology

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