Journal Article

Bradykinin stimulates the intracellular calcium activity in human mesothelial cells.

M Andre, S Rahimi, P Schollmeyer and H Pavenstädt

in Nephrology Dialysis Transplantation

Volume 13, issue 8, pages 2047-2052
Published in print August 1998 | ISSN: 0931-0509
Published online August 1998 | e-ISSN: 1460-2385 | DOI: http://dx.doi.org/10.1093/ndt/13.8.2047
Bradykinin stimulates the intracellular calcium activity in human mesothelial cells.

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AIM: The aim of this study was to investigate the influence of bradykinin on the intracellular calcium activity ([Ca2+]i) in human mesothelial cells in culture. RESULTS: Bradykinin (1-1000 nmol/l) caused a concentration-dependent and reversible increase of [Ca2+]i in mesothelial cells (n = 94); 10 nmol/l bradykinin increased [Ca2+]i from 23 +/- 9 to 670 +/- 170 nmol/l (n = 36). The pattern of the bradykinin-induced [Ca2+]i increase was biphasic with a transient [Ca2+]i peak, which was followed by a sustained [Ca2+]i plateau. The bradykinin-mediated [Ca2+]i plateau, but not the peak, was inhibited in a solution with an extracellular reduced Ca2+ concentration (from 1000 to 1 micromol/l, n = 11). Flufenamate (> or = 10 micromol/l), an inhibitor of non-selective ion channels abolished the bradykinin-mediated increase of [Ca2+], whereas the L-type Ca2+ channel blocker nicardipine (10 micromol/l) had no effect (n = 3-5). The [Ca2+]i response to bradykinin was inhibited by the BK2 antagonist Hoe 140 (IC50 +/- k7 nmol/l, n = 30). CONCLUSIONS: The data indicate that bradykinin stimulates [Ca2+]i in mesothelial cells by a release of Ca2+ from intracellular stores and an influx from Ca2+ through non-selective channels via a BK2 receptor.

Journal Article.  0 words. 

Subjects: Nephrology

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