Journal Article

PDGF-AB release during and after haemodialysis procedure

Giuseppe Cianciolo, Sergio Stefoni, Fulvia Zanchelli, Sandra Iannelli, Luigi Colì, Luigi Carlo Borgnino, Lucia Barbara De Sanctis, Vittario Stefoni, Antonio De Pascalis, Elisabetta Isola and Gaetano La Hanna

in Nephrology Dialysis Transplantation

Published on behalf of European Renal Association - European Dialysis and Transplant Assoc

Volume 14, issue 10, pages 2413-2419
Published in print October 1999 | ISSN: 0931-0509
Published online October 1999 | e-ISSN: 1460-2385 | DOI: http://dx.doi.org/10.1093/ndt/14.10.2413
PDGF-AB release during and after haemodialysis procedure

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Background. During haemodialysis blood–membrane contact causes the release of the content of platelet α-granules, which contain platelet-derived growth factor (PDGF). In view of its possible role in accelerated atherosclerotic processes, we evaluated the intra- and post-dialytic changes in PDGF-AB serum levels during haemodialysis sessions performed using a cellulosic membrane.

Methods. Using the ELISA method, PDGF-AB, platelet factor-4 (PF4) and β-thromboglobulin (β-TG) levels were determined in peripheral blood, as well as in arterial and venous haemodialyser lines, in 10 patients each of whom underwent five consecutive dialysis sessions with a CU membrane. Blood samples were taken at 0, 15, 30, 60, 120, 180 and 240 min during dialysis and at 1, 4 and 20 h after the end of the session. In the same group of patients the levels of the same molecules were also determined after a heparin bolus injection of 4500 IU, blood samples were taken at 0, 15 and 30 min after injection of the bolus.

Results. PDGF-AB serum levels increased, remained consistently high during the haemodialysis session (in particular +134±20% after 30 min, P<0.001, and +140±5% after 240 min, P<0.001) and returned to basal values only after 20 h following the end of the session. PF4 and β-TG showed a similar trend to PDGF. The heparin bolus injection caused only a small increase (+15±5% at 30 min) in PDGF-AB serum levels.

conclusions. PDGF-AB is released during dialysis mainly as consequence of the blood–membrane contact and it returns only slowly to basal values.

Keywords: accelerated atherosclerosis; dialysis; growth factors; platelet activation; platelet-derived growth factor

Journal Article.  4616 words.  Illustrated.

Subjects: Nephrology

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