Journal Article

Antibody‐induced modulation of the leukocyte CD11b integrin prevents mild but not major renal ischaemic injury

Luis C. F. Tajra, Xavier Martin, Jacqueline Margonari, Nelly Blanc‐Brunat, Michio Ishibashi, Geneviève Vivier, Jean P. Steghens, Hiroto Kawashima, Masayuki Miyasaka, Jean‐M. Dubernard and Jean‐P. Revillard

in Nephrology Dialysis Transplantation

Published on behalf of European Renal Association - European Dialysis and Transplant Assoc

Volume 15, issue 10, pages 1556-1561
Published in print October 2000 | ISSN: 0931-0509
Published online October 2000 | e-ISSN: 1460-2385 | DOI: http://dx.doi.org/10.1093/ndt/15.10.1556
Antibody‐induced modulation of the leukocyte CD11b integrin prevents mild but not major renal ischaemic injury

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Background. CD11/CD18 β2 integrins are involved in leukocyte adhesion to the activated endothelium, and therefore represent a possible therapeutic target in the prevention of ischaemic acute renal failure (ARF).

Methods. To assess the effect of an anti‐CD11b monoclonal antibody (mAb) in ischaemic ARF, uninephrectomized Fischer rats were subjected to 45 or 60 min of warm renal ischaemia, then received 1 mg of anti‐CD11b mAb 5 min before reperfusion.

Results. After 45 min of ischaemia, renal function tests at 24 and 48 h were less altered in mAb‐treated than in control rats, but after 60 min of ischaemia the same level of renal insufficiency was observed in the two groups. In parallel, milder tubular necrosis and less leukocyte infiltration were observed in the treated group after 45 min of ischaemia, but no difference was seen after 60 min compared to the control group. The mAb was detected on blood neutrophils up to 48 h after infusion and a marked down‐regulation of CD11b expression on neutrophil surfaces was documented by flow cytometry.

Conclusion. These results indicate that anti‐CD11b mAb administered prior to reperfusion decreases moderate ischaemic ARF but fails to prevent renal injury secondary to prolonged ischaemia in this model.

Keywords: adhesion molecules; integrins; Mac‐1; monoclonal antibody; rat; renal ischaemic injury

Journal Article.  3846 words.  Illustrated.

Subjects: Nephrology

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