Journal Article

Polymorphonuclear leukocyte rigidity is defective in patients with chronic renal failure

Athanasios T. Skoutelis, Vasilios E. Kaleridis, Dimitrios S. Goumenos, George M. Athanassiou, Yannis F. Missirlis, Jannis G. Vlachojannis and Harry P. Bassaris

in Nephrology Dialysis Transplantation

Published on behalf of European Renal Association - European Dialysis and Transplant Assoc

Volume 15, issue 11, pages 1788-1793
Published in print November 2000 | ISSN: 0931-0509
Published online November 2000 | e-ISSN: 1460-2385 | DOI: http://dx.doi.org/10.1093/ndt/15.11.1788
Polymorphonuclear leukocyte rigidity is defective in patients with chronic renal failure

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Background. The purpose of the study was to investigate the rigidity of polymorphonuclear leukocytes (PMNs) in non‐dialysed chronic renal failure (CRF) and haemodialysis (HD) patients.

Methods. PMN rigidity as well as tumour necrosis factor α (TNF‐α) and interleukin 1β (IL‐1β) plasma levels were assessed in 10 early‐stage CRF, 10 late‐stage non‐HD, and 10 HD patients, before and during dialysis. In HD patients both cellulose acetate and polysulphone membranes were used. Ten healthy subjects served as controls. Rigidity was tested by counting the deformability in morphologically passive PMNs by the micropipette method. Cytokine levels were measured by enzyme‐linked immunosorbent assay.

Results. PMN rigidity was significantly increased in end‐stage CRF patients regardless of HD but not in early‐stage CRF. In HD patients PMN rigidity increased significantly 60 min after initiation of HD. There was an increase of TNF‐α and IL‐1β levels in end‐stage non‐HD and HD patients and a further increase at 60 min after initiation of HD. The percentage of morphologically activated PMNs was increased only during dialysis. The nature of the HD membrane had no influence on rigidity, PMN activation, or cytokine production.

Conclusions. The results indicate that PMN rigidity is defective in end‐stage chronic CRF patients and is further increased 60 min after initiation of HD, regardless of the nature of the HD membrane used. PMN activation, increased TNF‐α and IL‐1β levels, or a direct PMN impairment may cause the observed cell rigidity.

Keywords: chronic renal failure; deformability; haemodialysis; polymorphonuclear leukocytes; rigidity; uraemia

Journal Article.  3533 words.  Illustrated.

Subjects: Nephrology

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