Journal Article

Inhibitory effects of rosmarinic acid on the proliferation of cultured murine mesangial cells

Toshiaki Makino, Takahiko Ono, Eri Muso, Haruyoshi Yoshida, Gisho Honda and Shigetake Sasayama

in Nephrology Dialysis Transplantation

Published on behalf of European Renal Association - European Dialysis and Transplant Assoc

Volume 15, issue 8, pages 1140-1145
Published in print August 2000 | ISSN: 0931-0509
Published online August 2000 | e-ISSN: 1460-2385 | DOI: http://dx.doi.org/10.1093/ndt/15.8.1140
Inhibitory effects of rosmarinic acid on the proliferation of cultured murine mesangial cells

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Background. Rosmarinic acid is a phenolic compound widely distributed in Labiatae herbs such as rosemary, sweet basil, and perilla, which are frequently used with meat and fish dishes in Western and Asian countries. In the present study we investigated the effects of rosmarinic acid on cultured murine mesangial cell proliferation.

Methods. Cultured murine mesangial cells were stimulated by growth factors with or without rosmarinic acid, and [3H]thymidine incorporation was measured in regard both to signal transduction and to cell cycle dependency. In other experiments, mRNA extracted from the cells was analysed by Northern blotting.

Results. Rosmarinic acid inhibited the cell proliferation induced by platelet‐derived growth factor (PDGF) (P<0.01; IC50 values, 1.4 μg/ml) or tumour necrosis factor‐α (P<0.01; IC50 values, 3.8 μg/ml), and these effects involved both the G0/G1 and G1/S phases of the cell cycle. Rosmarinic acid also suppressed the mRNA expressions of PDGF and c‐myc in PDGF‐stimulated mesangial cells.

Conclusions. Rosmarinic acid inhibits cytokine‐induced mesangial cell proliferation and suppresses PDGF and c‐myc mRNA expression in PDGF‐stimulated mesangial cells. Rosmarinic acid in Labiatae herbs might be a promising agent to prevent mesangial cell proliferation.

Keywords: glomerulonephritis; Labiatae herbs; mesangial cell proliferation; rosmarinic acid

Journal Article.  3270 words.  Illustrated.

Subjects: Nephrology

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