Journal Article

Identification of cellular origin of type I collagen in glomeruli of rats with crescentic glomerulonephritis induced by anti‐glomerular basement membrane antibody

Jin‐Song He, Kayo Hayashi, Satoshi Horikoshi, Kazuhiko Funabiki, Isao Shirato and Yasuhiko Tomino

in Nephrology Dialysis Transplantation

Published on behalf of European Renal Association - European Dialysis and Transplant Assoc

Volume 16, issue 4, pages 704-711
Published in print April 2001 | ISSN: 0931-0509
Published online April 2001 | e-ISSN: 1460-2385 | DOI: http://dx.doi.org/10.1093/ndt/16.4.704
Identification of cellular origin of type I collagen in glomeruli of rats with crescentic glomerulonephritis induced by anti‐glomerular basement membrane antibody

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Background. Type I collagen is an interstitial collagen, which is not present in normal glomeruli. As type I collagen was observed in advanced glomerular lesions, it appears to be associated with deterioration of renal function. However, the origins of cells expressing type I collagen mRNA in glomeruli of diseased kidneys remains controversial.

Methods. We examined the expression of type I collagen in glomeruli at protein and mRNA levels in rat crescentic glomerulonephritis induced by anti‐glomerular basement membrane (GBM) antibody. In addition, in situ hybridization and immunohistochemical staining of serial sections were performed to identify the cellular origin of type I collagen in glomeruli.

Results. Semi‐quantitative reverse transcription‐polymerase chain reaction (RT‐PCR) in isolated glomeruli showed that mRNA expression of type I collagen was remarkably increased on days 7, 14, and 28 after anti‐GBM antibody injection (12.2±1.4, 20.2±2.1 and 14.6±1.0‐fold over day 0, respectively). Immunofluorescence for type I collagen demonstrated marked staining in the fibrocellular and fibrous crescents, and weak staining within glomerular mesangial areas. In close association with mRNA levels analysed by RT‐PCR, in situ hybridization revealed predominant presence of α1(I) collagen mRNA in cells within crescentic areas and Bowman's capsules. Serial section analysis for immunostaining and in situ hybridization showed that some α1(I) collagen mRNA‐positive cells were also positive for cytokeratin. In contrast, no α1(I) collagen mRNA‐positive cells were stained by ED‐1 and podocalyxin.

Conclusions. It appears that increased expression of type I collagen at the protein and mRNA levels in glomeruli is involved in the progression of glomerulonephritis. At least in this crescentic model, parietal epithelial cells (PECs) may partially contribute to the dysregulated production of type I collagen, which leads to glomerulosclerosis.

Keywords: crescentic glomerulonephritis; cytokeratin; in situ hybridization; parietal epithelial cell; type I collagen

Journal Article.  3682 words.  Illustrated.

Subjects: Nephrology

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