Journal Article

Reduction of glomerular hyperfiltration by dopamine D<sub>2</sub>‐like receptor blockade in experimental diabetes mellitus

Gerd Luippold, Martina Beilharz and Bernd Mühlbauer

in Nephrology Dialysis Transplantation

Published on behalf of European Renal Association - European Dialysis and Transplant Assoc

Volume 16, issue 7, pages 1350-1356
Published in print July 2001 | ISSN: 0931-0509
Published online July 2001 | e-ISSN: 1460-2385 | DOI: http://dx.doi.org/10.1093/ndt/16.7.1350
Reduction of glomerular hyperfiltration by dopamine D2‐like receptor blockade in experimental diabetes mellitus

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Background. Dopamine D2‐like receptors are involved in the physiological response of renal haemodynamics to amino‐acid infusion. The present study was performed to investigate whether domperidone, a D2‐like receptor antagonist, modulates the pathological hyperfiltration in experimental diabetes mellitus.

Methods. Renal function was studied in anaesthetized rats 2 weeks after induction of moderate diabetes mellitus by streptozotocin, and in non‐diabetic controls. Rats in both groups continuously received domperidone or vehicle via drinking water. Following infusion of Ringer's saline for measurement of baseline values, an i.v. amino‐acid load was applied to investigate the renal functional reserve.

Results. In vehicle‐treated diabetic rats baseline glomerular filtration rate and renal plasma flow were significantly higher compared with controls (1.10± 0.04 vs 0.83±0.02 (P<0.004) and 4.83±0.26 vs 3.32±0.24 ml/min/100 g body weight (bw) (P<0.001) respectively). Domperidone completely normalized glomerular filtration rate and renal plasma flow in diabetic rats to values observed in vehicle‐treated controls (0.81±0.07 (P=0.740) and 3.35± 0.30 ml/min/100 g bw (P=0.889) respectively). In the clearance experiments, arterial blood pressure, urinary flow rate and sodium excretion did not significantly differ when comparing the four groups. However, in conscious rats, urinary flow rate and sodium excretion were significantly higher in diabetic rats compared with non‐diabetic controls. In both non‐diabetic groups, amino‐acid infusion induced a significant glomerular hyperfiltration that was completely absent in diabetic rats, and restored by domperidone treatment. In conscious vehicle‐treated diabetic rats urinary albumin excretion was enhanced (449.0±47.7 vs 185.7± 18.1 μg/24 h in non‐diabetic rats (P<0.001)) and significantly lowered in diabetic rats by domperidone treatment (109.8±15.4 μg/24 h (P<0.001)).

Conclusion. The data suggest that dopaminergic mechanisms are involved in the changes in renal haemodynamics during early experimental diabetes mellitus in rats.

Keywords: D2‐like receptors; diabetes mellitus; dopamine; nephropathy; rats; renal haemodynamics

Journal Article.  4202 words.  Illustrated.

Subjects: Nephrology

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