Journal Article

Reduced 11β‐hydroxysteroid dehydrogenase activity in experimental nephrotic syndrome

Bruno Vogt, Bernhard Dick, Hans‐Peter Marti, Felix J. Frey and Brigitte M. Frey

in Nephrology Dialysis Transplantation

Published on behalf of European Renal Association - European Dialysis and Transplant Assoc

Volume 17, issue 5, pages 753-758
Published in print May 2002 | ISSN: 0931-0509
Published online May 2002 | e-ISSN: 1460-2385 | DOI: http://dx.doi.org/10.1093/ndt/17.5.753
Reduced 11β‐hydroxysteroid dehydrogenase activity in experimental nephrotic syndrome

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Background. The disease state of the nephrotic syndrome is characterized by abnormal renal sodium retention that cannot be completely explained by a secondary hyperaldosteronism for the following reasons. Firstly, in rats an enhanced sodium retention is observed before proteinuria with intravascular volume depletion occurs. Secondly, in patients with the nephrotic syndrome, volume expansion with hypertension has been reported despite suppression of the renin‐aldosterone system. Therefore, another mechanism for sodium retention must be postulated for this disease state. We hypothesize that this mechanism is a reduced 11β‐hydroxysteroid dehydrogenase 2 (11β‐HSD2) activity, a phenomenon known to cause enhanced access of cortisol or corticosterone to the mineralocorticoid receptor.

Methods. We assessed the 11β‐HSD activity by measuring the urinary ratio of tetrahydrocorticosterone (THB) plus 5α‐tetrahydrocorticosterone (5α‐THB) to 11‐dehydro‐tetrahydrocorticosterone (THA) by gas chromatography–mass spectrometry in rats with puromycin aminonucleoside (PAN)‐induced proteinuria and with adriamycin nephrosis. Furthermore, the plasma ratios of corticosterone to 11‐dehydrocorticosterone were measured.

Results. The urinary ratio of (THB+5α‐THB)/THA increased in all animals following injection of PAN or adriamycin, indicating a reduced activity of 11β‐HSD. The reduced activity of 11β‐HSD was confirmed by an increased plasma ratio of corticosterone to 11‐dehydrocorticosterone. The changes in the glucocorticoid metabolite ratios were already present before significant proteinuria appeared.

Conclusion. PAN‐ or adriamycin‐treated rats develop proteinuria with a reduced activity of 11β‐HSD, a mechanism contributing to the abnormal sodium retention in nephrotic syndrome.

Keywords: adriamycin; gas chromatography–mass spectrometry; 11β‐hydroxysteroid dehydrogenase; nephrotic syndrome; proteinuria; puromycin aminonucleoside; sodium retention

Journal Article.  3602 words.  Illustrated.

Subjects: Nephrology

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