Journal Article

Cadherin and catenin staining in podocytes in development and puromycin aminonucleoside nephrosis

Eishin Yaoita, Nobuo Sato, Yutaka Yoshida, Masaaki Nameta and Tadashi Yamamoto

in Nephrology Dialysis Transplantation

Published on behalf of European Renal Association - European Dialysis and Transplant Assoc

Volume 17, issue suppl_9, pages 16-19
Published in print September 2002 | ISSN: 0931-0509
Published online September 2002 | e-ISSN: 1460-2385 | DOI: http://dx.doi.org/10.1093/ndt/17.suppl_9.16
Cadherin and catenin staining in podocytes in development and puromycin aminonucleoside nephrosis

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Podocytes have been shown to express classical cadherins in the early stage of glomerulogenesis, but it remains unclear whether podocytes in normal and pathological conditions express cadherins in the adult stage. To address this question, rat podocytes in the neonate, the adult and in puromycin aminonucleoside (PAN) nephrosis were examined by immunofluorescence microscopy using antibodies against α‐ and β‐catenins and anti‐pan‐cadherin antibody, and by immunoelectron microscopy using anti‐α‐catenin antibody. In the neonate, all the antibodies reacted with the junctional complex of podocytes in the S‐shaped body stage. The staining disappeared during the capillary loop stage, when foot processes and slit diaphragms are formed. In the adult, podocytes were not stained with any of the antibodies, whereas distinct staining with the antibodies was detected in endothelial cells and mesangial cells in the glomerulus. These findings were identical in kidneys of PAN nephrosis; podocytes did not show any significant staining. These results indicate that the composition of intercellular junction molecules of podocytes in the adult is different from that of immature podocytes even under the pathological condition.

Keywords: cadherin; catenin; glomerular visceral epithelial cell; podocyte; puromycin aminonucleoside

Journal Article.  0 words. 

Subjects: Nephrology

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