Journal Article

Inflamed site‐specific delivery of bone marrow‐derived cells carrying IL‐1Ra

Takashi Yokoo

in Nephrology Dialysis Transplantation

Published on behalf of European Renal Association - European Dialysis and Transplant Assoc

Volume 17, issue suppl_9, pages 91-93
Published in print September 2002 | ISSN: 0931-0509
Published online September 2002 | e-ISSN: 1460-2385 | DOI: http://dx.doi.org/10.1093/ndt/17.suppl_9.91
Inflamed site‐specific delivery of bone marrow‐derived cells carrying IL‐1Ra

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A gene delivery system using bone marrow‐derived CD11b+CD18+ cells and their interaction with adhesion molecules was established. After transplantation into mice, these vehicle cells may be recruited into glomeruli upon lipopolysaccharide (LPS) treatment, and the number of recruited cells corresponds to the expression of intercellular adhesion molecule‐1 (ICAM‐1) in glomeruli. Using this system, interleukin‐1 receptor antagonist (IL‐1Ra) was delivered into animal models of glomerulonephritis evoked by anti‐glomerular basement membrane antibody (anti‐GBM nephritis). Urinary albumin excretion and the serum creatinine level were significantly elevated after anti‐GBM antibody injection in mock‐treated mice, whereas they were suppressed in the IL‐1Ra‐treated mice. Histological analysis revealed that glomerular injuries were also suppressed in IL‐1Ra‐treated mice. We further confirmed that specificity for inflamed glomeruli was significantly enhanced by a combination of the Cre/loxP system with the IL‐1β promoter. These data suggested that our novel system may be used as a therapeutic intervention for glomerulonephritis.

Keywords: adhesion molecule; bone marrow transplantation; Cre/loxP system; glomerulonephritis

Journal Article.  0 words. 

Subjects: Nephrology

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