Journal Article

Increased plasma <i>S</i>‐nitrosothiol levels in chronic haemodialysis patients

Ziad A. Massy, Didier Borderie, Thao Nguyen‐Khoa, Tilman B. Drüeke, Ohvanesse G. Ekindjian and Bernard Lacour

in Nephrology Dialysis Transplantation

Published on behalf of European Renal Association - European Dialysis and Transplant Assoc

Volume 18, issue 1, pages 153-157
Published in print January 2003 | ISSN: 0931-0509
Published online January 2003 | e-ISSN: 1460-2385 | DOI: http://dx.doi.org/10.1093/ndt/18.1.153
Increased plasma S‐nitrosothiol levels in chronic haemodialysis patients

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Background. An impairment of nitric oxide (NO) bioavailability and/or metabolism may contribute to the excessive incidence of atherosclerotic complications observed in haemodialysis (HD) patients. Recent evidence indicates that NO metabolism involves a family of NO‐related molecules that have not yet been explored in such patients. The aim of our study was to determine the plasma levels of S‐nitrosothiol and nitrotyrosine in chronic HD patients, and to evaluate potential factors influencing their levels.

Methods. Plasma levels of S‐nitrosothiols and nitrotyrosine were determined in 22 non‐smoking HD patients and 12 healthy control subjects, together with albumin, homocysteine, haemoglobin, highly sensitive C‐reactive protein (hsCRP) and various components of the oxidant–antioxidant system at the plasma and erythrocyte levels.

Results. While plasma nitrosothiol levels were significantly higher in HD patients than in controls (2.25±1.17 vs 0.45±0.45 µmol/l, respectively, P<0.0001), nitrotyrosine levels were not different. HD patients also exhibited a marked deficit of ascorbate and low plasma glutathione peroxidase activity. An inverse relationship was found between plasma S‐nitrosothiol and blood haemoglobin in HD patients (P<0.005). No direct relationship was observed between plasma S‐nitrosothiol levels and any of the oxidative stress markers, or hsCRP levels.

Conclusion. This study demonstrates high plasma S‐nitrosothiol levels in HD patients, which are partially related to low blood haemoglobin concentrations. The pathophysiological significance of this elevation remains to be elucidated. A possible protective role against nitrosative stress is suggested in presence of normal plasma nitrotyrosine levels in such patients.

Keywords: atherosclerosis; haemodialysis; nitric oxide; nitrotyrosine; oxidant–antioxidant system; S‐nitrosothiol

Journal Article.  3417 words.  Illustrated.

Subjects: Nephrology

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