Journal Article

Over‐expression of platelet‐derived growth factor in human diabetic nephropathy

Robyn G. Langham, Darren J. Kelly, Julie Maguire, John P. Dowling, Richard E. Gilbert and Napier M. Thomson

in Nephrology Dialysis Transplantation

Published on behalf of European Renal Association - European Dialysis and Transplant Assoc

Volume 18, issue 7, pages 1392-1396
Published in print July 2003 | ISSN: 0931-0509
Published online July 2003 | e-ISSN: 1460-2385 | DOI: http://dx.doi.org/10.1093/ndt/gfg177
Over‐expression of platelet‐derived growth factor in human diabetic nephropathy

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Background. The pathogenetic mechanisms responsible for progressive renal impairment of diabetic nephropathy are still poorly understood, despite its growing incidence. Increasing evidence suggests that growth factors may contribute to the initiation and progressive fibrosis of diabetic nephropathy. In this study, the gene expression and protein distribution of platelet‐derived growth factor‐A and ‐B (PDGF‐A and PDGF‐B) in human diabetic nephropathy were examined.

Methods. PDGF‐A and PDGF‐B mRNA levels in surplus renal biopsy tissue from seven patients with overt diabetic nephropathy and six nephrectomy samples were examined using quantitative reverse transcription–polymerase chain reaction (RT–PCR). In addition, each sample was also examined immunohistochemically to quantify and localize peptide expression of each PDGF isoform.

Results. Gene expression of PDGF‐A and PDGF‐B mRNA were increased 22‐ and 6‐fold, respectively, in biopsies from patients with diabetic nephropathy compared with control tissue. Immunostaining also demonstrated increased peptide expression of both PDGF‐A and PDGF‐B in diabetic nephropathy, with each isoform showing a specific pattern of tissue distribution.

Conclusions. The findings of increased gene and protein expression of PDGF in renal biopsies from patients with diabetic nephropathy imply a potential role for this prosclerotic growth factor in the development of the progressive fibrosis that characterizes human diabetic kidney disease.

Keywords: diabetic nephropathy; platelet‐derived growth factor; RT–PCR

Journal Article.  2431 words.  Illustrated.

Subjects: Nephrology

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