Journal Article

Hypoalbuminaemia enhances the renal vasoconstrictor effect of lysophosphatidylcholine

Thi Danh Vuong, Branko Braam, Nel Willekes-Koolschijn, Peter Boer, Hein A. Koomans and Jaap A. Joles

in Nephrology Dialysis Transplantation

Published on behalf of European Renal Association - European Dialysis and Transplant Assoc

Volume 18, issue 8, pages 1485-1492
Published in print August 2003 | ISSN: 0931-0509
Published online August 2003 | e-ISSN: 1460-2385 | DOI: http://dx.doi.org/10.1093/ndt/gfg238
Hypoalbuminaemia enhances the renal vasoconstrictor effect of lysophosphatidylcholine

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Background. Lysophosphatidylcholine (LPC) causes vascular dysfunction in vitro. Lipoprotein LPC is increased in hypoalbuminaemia. Albumin binds LPC and restores LPC-induced abnormalities. We hypothesized that in vivo LPC impairs blood flow more in hypoalbuminaemia than in normoalbuminaemia.

Methods. Increasing concentrations of LPC were infused intra-renally in Nagase analbuminaemic rats (NAR) and Sprague–Dawley rats (controls).

Results. Intra-renal LPC (0.1 µmol/min, 20 min) reduced renal blood flow (RBF) more (P < 0.01) in NAR (from 8.3 ± 0.3 to 4.0 ± 1.1) than in controls (from 7.7 ± 0.7 to 5.8 ± 0.5 ml/min/g kidney). Lysophosphatidylethanolamine had no effect. After stopping LPC, RBF recovery was delayed in NAR [median 90 (range: 70–90) vs 45 min (40–60), P < 0.01]. Intravenous bovine serum albumin (BSA) prevented LPC-induced vasoconstriction in both strains. Prolonging LPC for 60 min delayed recovery of RBF. In this setting, intra-renal BSA completely restored RBF in 75 min (30–90), while intra-renal saline over 75 min only resulted in 33 ± 13% recovery (P < 0.01). Baseline renal LPC content was unchanged in NAR. However, intra-renal LPC infusion doubled renal LPC content in NAR, but had no effect in controls.

Conclusions. In NAR, baseline RBF and renal LPC content are normal. However, exposure of NAR to LPC results in much more vasoconstriction and accumulation of LPC than in normoalbuminaemia. Addition of albumin prevents and restores LPC-induced vasoconstriction.

Keywords: albumin; analbuminaemic rat; lysophosphatidylcholine; hypoalbuminaemia; renal blood flow

Journal Article.  5094 words.  Illustrated.

Subjects: Nephrology

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