Journal Article

Age-dependent thickening of glomerular basement membrane has no major effect on glomerular hydraulic conductivity

Klaus H. Neumann, Christian Kellner, Karlwilhelm Kühn, Hilmar Stolte and Hans-Joachim Schurek

in Nephrology Dialysis Transplantation

Published on behalf of European Renal Association - European Dialysis and Transplant Assoc

Volume 19, issue 4, pages 805-811
Published in print April 2004 | ISSN: 0931-0509
Published online April 2004 | e-ISSN: 1460-2385 | DOI:
Age-dependent thickening of glomerular basement membrane has no major effect on glomerular hydraulic conductivity

Show Summary Details


Background. The effect of the increasing thickness of the glomerular basement membrane (GBM), which is seen in ageing rats, on the effective hydraulic conductivity (k) of the glomerular capillary wall was studied in Wistar rats aged 2 and 18 months.

Methods. With the use of micropuncture techniques, ultrafiltration characteristics of cortical glomeruli were determined in isolated cell-free perfused kidneys. Because the filtration fraction in this preparation is low (3%) as a consequence of high perfusion rates at glomerular filtration rates comparable with in vivo conditions, uniform ultrafiltration conditions are provided over the whole filtering surface. After fixation at a defined perfusion pressure, the surface of glomerular capillaries (S) was obtained morphometrically on light microscopic sections of the glomeruli studied previously.

Results. The glomerular ultrafiltration coefficient (Kf) was 0.025 nl/s·mmHg in young rats and 0.038 nl/s·mmHg in old rats (P<0.0005) and S was 0.140 mm2 in young and 0.244 mm2 in old rats (P<0.0005). However, k was not significantly different (18.0 nl/s·mmHg·cm2 in young and 15.8 nl/s·mmHg·cm2 in old rats) despite a 2.4-fold increase of GBM thickness as estimated from electron microscopic sections.

Conclusions. These findings indicate that the age-dependent increase of GBM thickness in rat kidneys did not substantially increase hydraulic resistance of the glomerular capillary wall.

Keywords: ageing rat kidney; glomerular capillary basement membrane thickness; glomerular capillary surface area; glomerular effective hydraulic conductivity; isolated perfused kidney; micropuncture methods

Journal Article.  4450 words.  Illustrated.

Subjects: Nephrology

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.