Journal Article

Evaluation of long-term transport ability of a bioartificial renal tubule device using LLC-PK<sub>1</sub> cells

Nazira Ozgen, Masuo Terashima, Tun Aung, Yoshinobu Sato, Chie Isoe, Takatoshi Kakuta and Akira Saito

in Nephrology Dialysis Transplantation

Published on behalf of European Renal Association - European Dialysis and Transplant Assoc

Volume 19, issue 9, pages 2198-2207
Published in print September 2004 | ISSN: 0931-0509
Published online July 2004 | e-ISSN: 1460-2385 | DOI: http://dx.doi.org/10.1093/ndt/gfh399
Evaluation of long-term transport ability of a bioartificial renal tubule device using LLC-PK1 cells

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Background. Haemodialysis therapy does not provide renal tubule function, such as active fluid and solute transport, nor metabolic or endocrine action. Moreover, this treatment is usually associated with serious complications and high mortality. We constructed a bioartificial renal tubule device by using renal tubule epithelial cells in an artificial membrane, and evaluated transport properties of the device for 2 weeks.

Methods. A renal epithelial cell line, LLC-PK1 (Lewis-lung cancer porcine kidney), was seeded on polysulfone hollow fibres in small and large modules. We studied perfusion and leakage of urea nitrogen (UN) and creatinine (Cr), as well as reabsorption of water, glucose and sodium for a period of 2 weeks.

Results. Cell-lined hollow fibre membranes significantly reduced the leakage of UN and Cr throughout the 2 week period. Reabsorption of water, glucose and sodium were adequate from days 3 to 10 and gradually decreased thereafter. LLC-PK1 cells actively transported these substances. Scanning electron microscopy revealed that cells in the hollow fibres on day 8 became completely confluent. However, they became multi-layered and almost obstructed the hollow fibres on day 13.

Conclusions. This bioartificial renal tubule device functioned to reabsorb water, glucose and sodium for ∼10 days. This is the first report of successful long-term evaluation of a bioartificial renal tubule device. This device, in combination with continuous haemofiltration, may provide treatment to prevent complications of dialysis and raise the quality of life in chronic renal failure patients.

Keywords: bioartificial renal tubule device; chronic renal failure; continuous haemofiltration; LLC-PK1 cells

Journal Article.  5866 words.  Illustrated.

Subjects: Nephrology

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