Journal Article

Nonerythropoietic derivative of erythropoietin protects against tubulointerstitial injury in a unilateral ureteral obstruction model

Harumi Kitamura, Yoshitaka Isaka, Yoshitsugu Takabatake, Ryoichi Imamura, Chigure Suzuki, Shiro Takahara and Enyu Imai

in Nephrology Dialysis Transplantation

Published on behalf of European Renal Association - European Dialysis and Transplant Assoc

Volume 23, issue 5, pages 1521-1528
Published in print May 2008 | ISSN: 0931-0509
Published online January 2008 | e-ISSN: 1460-2385 | DOI: http://dx.doi.org/10.1093/ndt/gfm842
Nonerythropoietic derivative of erythropoietin protects against tubulointerstitial injury in a unilateral ureteral obstruction model

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Background. Erythropoietin (EPO), a member of the cytokine type I superfamily, acts to increase circulating erythrocytes primarily by preventing apoptosis of erythroid progenitors, is known to protect tissues and can raise haemoglobin (Hb) concentrations. Recently, a second receptor for EPO comprising the EPO receptor and β-common receptor has been reported to mediate EPO-induced tissue protection. EPO modified by carbamylation (CEPO) only signals through this second receptor. Accordingly, we hypothesized that treatment with CEPO, which would not increase Hb concentrations, would protect against tubular damage and thereby inhibit tubulointerstitial injuries.

Methods. We evaluated therapeutic effects of CEPO using a rat unilateral ureteral obstruction model.

Results. CEPO decreased tubular apoptosis and α-smooth muscle actin (αSMA) expression in the absence of polycythaemia, while the untreated obstructed kidneys exhibited increased tubular apoptosis with expanded (αSMA) expression. While EPO treatment similarly inhibited tubular apoptosis and αSMA expression, EPO treatment increased Hb concentrations and induced a wedge-shaped infarction.

Conclusion. We established a therapeutic approach using CEPO to protect against tubulointerstitial injury. The therapeutic value of this approach warrants further attention and preclinical studies.

Keywords: apoptosis; carbamylated erythropoietin; tubulointerstitial injury; ureteral obstruction

Journal Article.  4446 words.  Illustrated.

Subjects: Nephrology

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