Journal Article

Amelioration of diabetic tubulointerstitial damage in liver-type fatty acid-binding protein transgenic mice

Atsuko Kamijo-Ikemori, Takeshi Sugaya, Ayako Sekizuka, Kazuaki Hirata and Kenjiro Kimura

in Nephrology Dialysis Transplantation

Published on behalf of European Renal Association - European Dialysis and Transplant Assoc

Volume 24, issue 3, pages 788-800
Published in print March 2009 | ISSN: 0931-0509
Published online October 2008 | e-ISSN: 1460-2385 | DOI: http://dx.doi.org/10.1093/ndt/gfn573
Amelioration of diabetic tubulointerstitial damage in liver-type fatty acid-binding protein transgenic mice

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Background. Renoprotection of liver-type fatty acid binding protein (L-FABP) was demonstrated in a streptozotocin (STZ)-induced diabetic mouse model.

Methods. Established human L-FABP (hL-FABP) transgenic (Tg) mice and wild-type (WT) mice were divided into two groups: diabetic mice were uninephrectomized and injected with STZ; control mice were uninephrectomized and injected with a citrate buffer alone. Although mouse L-FABP was not expressed in WT mice, hL-FABP was expressed in the proximal tubules of the diabetic Tg mice and in the control Tg mice at 8 and 14 weeks after these injections.

Results. The expression of renal hL-FABP increased significantly in diabetic Tg mice compared to control Tg mice. A number of macrophages (F4/80) infiltrating the interstitium, the gene expressions of MCP-1, MCP-3, TGF-β, Fas, Bax and RAGE were significantly lower in diabetic Tg kidneys compared with diabetic WT kidneys. In the diabetic Tg kidneys, the degree of the tubulointerstitial injury and the deposition of type IV collagen were significantly lower than that of diabetic WT kidneys. The expressions of catalase and glutathione peroxidase-1 were significantly lower in diabetic Tg kidneys compared with diabetic WT kidneys.

Conclusions. Renal L-FABP ameliorated the tubulointerstitial damage of type 1 diabetic mice.

Keywords: diabetic nephropathy; liver-type fatty acid binding protein; proximal tubule; tubulointerstitial damage

Journal Article.  6954 words.  Illustrated.

Subjects: Nephrology

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