Journal Article

IF/TA-related metabolic changes—proteome analysis of rat renal allografts

Stefan Reuter, Stefanie Reiermann, Reka Wörner, Rita Schröter, Bayram Edemir, Fritz Buck, Stefanie Henning, Jasna Peter-Katalinic, Beate Vollenbröker, Kerstin Amann, Hermann Pavenstädt, Eberhard Schlatter and Gert Gabriëls

in Nephrology Dialysis Transplantation

Published on behalf of European Renal Association - European Dialysis and Transplant Assoc

Volume 25, issue 8, pages 2492-2501
Published in print August 2010 | ISSN: 0931-0509
Published online February 2010 | e-ISSN: 1460-2385 | DOI:
IF/TA-related metabolic changes—proteome analysis of rat renal allografts

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Background. Chronic allograft nephropathy, now more specifically termed interstitial fibrosis and tubular atrophy without evidence of any specific aetiology (IF/TA), is still an important cause of late graft loss. There is no effective therapy for IF/TA, in part due to the disease's multifactorial nature and its incompletely understood pathogenesis.

Methods. We used a differential in-gel electrophoresis and mass spectrometry technique to study IF/TA in a renal transplantation model. Dark Agouti (DA) kidneys were allogeneically transplanted to Wistar–Furth (DA–WF, aTX) rats. Syngeneic grafts (DA–DA, sTX) served as controls. Nine weeks after transplantation, blood pressure, renal function and electrolytes were studied, in addition to real-time PCR, western blot analysis, histology and immunohistochemistry.

Results. In contrast to sTX, the aTX developed IF/TA-dependent renal damage. Ten differentially regulated proteins were identified by 2D gel analysis and mass spectrometry, whereupon five proteins are mainly related to oxidative stress (aldo–keto reductase, peroxiredoxin-1, NAD+-dependent isocitrate dehydrogenase, iron-responsive element-binding protein-1 and serum albumin), two participate in cytoskeleton organization (l-plastin and ezrin) and three are assigned to metabolic functions (creatine kinase, ornithine aminotransferase and fructose-1,6-bisphosphatase).

Conclusion. The proteins related to IF/TA and involved in oxidative stress, cytoskeleton organization and metabolic functions may correspond with novel therapeutic targets.

Keywords: IF/TA; kidney; metabolism; proteomics

Journal Article.  6441 words.  Illustrated.

Subjects: Nephrology

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