Journal Article

Are <i>ex vivo</i> mesothelial cells representative of the <i>in vivo</i> transition from epithelial-to-mesenchymal cells in peritoneal membrane?

Ji-Hyung Cho, Jun-Young Do, Eun-Joo Oh, Hye-Myung Ryu, So-Young Park, San-Ok Kim, Seung-Hyea Hyun, Hye-Jin Seo, Gun-Hyun Kim, Ji-Young Choi, Chan-Duck Kim, Sun-Hee Park and Yong-Lim Kim

in Nephrology Dialysis Transplantation

Published on behalf of European Renal Association - European Dialysis and Transplant Assoc

Volume 27, issue 5, pages 1768-1779
Published in print May 2012 | ISSN: 0931-0509
Published online October 2011 | e-ISSN: 1460-2385 | DOI: http://dx.doi.org/10.1093/ndt/gfr604
Are ex vivo mesothelial cells representative of the in vivo transition from epithelial-to-mesenchymal cells in peritoneal membrane?

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Background.

We investigated whether ex vivo mesothelial cells found in peritoneal dialysis (PD) effluents were representative of the in vivo epithelial-to-mesenchymal transition (EMT) in peritoneal membrane.

Methods.

Thirty-six male Sprague–Dawley rats were equally divided into three groups: Group C (control), no PD; Group D, infused with 4.25% Dianeal and Group P, infused with 4.25% Physioneal. PD infusions (25 mL) were given twice daily for 8 weeks. The in vivo study included morphometric analyses performed on the peritoneal membranes of tissue specimens obtained at the end of the study. The ex vivo study included peritoneal mesothelial cells collected from PD effluent and cultured to confluence. Cells were scored with light microscopy.

Results.

PD for 8 weeks induced significant EMT. The in vivo expression of EMT markers (α-smooth muscle actin:E-cadherin ratio, matrix metalloproteinase-2 and Snail) was higher in Group D than in Group P. However, ex vivo EMT marker expression was similar in cells derived from Groups D and P. A significant correlation was observed among in vivo EMT markers. Moreover, the ex vivo cell score increased with time on PD. However, changes in the ex vivo cell score did not correlated with changes in the in vivo EMT marker expression. Furthermore, we found no correlation between ex vivo and in vivo cells in the expression of EMT markers.

Conclusions.

In this animal study, ex vivo findings did not reflect the in vivo EMT changes in the peritoneum. It may be necessary to improve the current methodology for ex vivo studies.

Keywords: epithelial-to-mesenchymal transition; peritoneal dialysis; peritoneal membrane

Journal Article.  5967 words.  Illustrated.

Subjects: Nephrology

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