A glycolytic enzyme (fructose-1,6-bisphosphate aldolase, EC188.8.131.52, 363 aa) that catalyses the conversion of fructose 1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. There are three isozymes, aldolases A, B, and C, with tissue-specific distributions. Aldolase, like phosphofructokinase, enolase, and hexokinase, binds actin filaments, probably to cause local concentrations of enzymes and their substrates. The glucose transporter GLUT4, is connected to actin via aldolase. Aldolase A deficiency is an autosomal recessive disorder associated with hereditary haemolytic anaemia, the enzyme being very thermolabile. Mutations in aldolase B are associated with fructose intolerance.