Barfoed's test

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A biochemical test to detect monosaccharide (reducing) sugars in solution, devised by the Swedish physician C. T. Barfoed (1815–99). Barfoed's reagent, a mixture of ethanoic (acetic) acid and copper(II) acetate, is added to the test solution and boiled. If any reducing sugars are present a red precipitate of copper(II) oxide is formed. The reaction will be negative in the presence of disaccharide sugars as they are weaker reducing agents.

Subjects: Chemistry — Biological Sciences.

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