biotinylated DNA

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DNA probes labeled with biotin (q.v.). Biotinylated deoxyuridine triphosphate is incorporated into the molecule by nick translation (q.v.). The probe is then hybridized to the specimen, such as denatured polytene chromosomes on a slide. The location of the biotin is visualized by complexing it with a streptavidin (q.v.) molecule that is attached to a color-generating agent. The technique is less time consuming than autoradiography and gives greater resolution. See Chronology, 1981, Langer et al.

Subjects: Genetics and Genomics.

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