Overview

Bradford assay


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A standard protein assay based on the change in absorbance of Coomassie Brilliant Blue G-250 when it binds to arginine and aromatic residues in the protein. It is important to standardize the assay with a protein of comparable arginine content, and for proteins with few aromatic residues (particularly collagen) the results are very inaccurate.

Subjects: Medicine and Health.


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