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cassette mechanism


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A mechanism for interchanging genes, e.g. the a and α genes that determine mating type in yeast. A double-stranded nuclease makes a cut at a specific point in the MAT (mating type) locus and the old gene is replaced with a copy of a silent gene (a different cassette) from one or other flanking regions, and the new copy becomes active. Similar mechanisms are used in some cloning vectors.

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