cassette mutagenesis

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A technique that involves removing from a gene a stretch of DNA flanked on either end by a restriction site (q.v.) and then inserting in its place a new DNA segment. This cassette can contain base substitutions or deletions at specific sites, and the phenotypic effects that result give insight into relative importance of specific subsegments of the region to the functioning of the gene or its product.

Subjects: Genetics and Genomics.

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