Single-stranded, complementary DNA produced from an RNA template by the action of RNA-dependent, DNA polymerase (reverse transcriptase) in vitro. If the RNA template has been processed to remove the introns, the cDNA will be much shorter than the gene from which the RNA was transcribed. The single-stranded, cDNA molecule may subsequently serve as a template for a DNA polymerase. The symbol cDNA is sometimes also applied to the double-stranded DNA molecule that results. See posttranscriptional processing.
Subjects: Genetics and Genomics — Medicine and Health.