chromatin immunoprecipitation

Quick Reference

A technique for identifying a specific DNA sequence in the genome (q.v.) to which a particular protein binds in vivo (q.v.). In this procedure, the protein in question is cross-linked to DNA with formaldehyde in vivo. The DNA is then extracted from cells and sheared into small fragments. Antibodies against the bound protein are then used to isolate the protein-DNA complex, the protein is released, and the polymerase chain reaction (PCR) (q.v.) is used to amplify the DNA sequence to which the protein was bound. The amplified DNA can then be identified by sequence analysis.

Subjects: Chemistry — Genetics and Genomics.

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