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An analytical technique for separating the components of complex mixtures, based on their repetitive distribution between a mobile phase (of gas or liquid) and a stationary phase (of solids or liquid-coated solids). The distribution of the different component molecules between the two phases is dependent on the method of chromatography used (e.g. gel-filtration, or ion-exchange), and on the movement of the mobile phase (which results in the differential migration and therefore separation of the components along the stationary phase).

Subjects: Biological Sciences.

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